CLIA Kit for Thyroid Stimulating Hormone (TSH)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78.1-20,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Canis familiaris; Canine (Dog)

Sensitivity

The minimum detectable dose of this kit is typically less than 28.5pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Thyroid Stimulating Hormone (TSH). A competitive inhibition reaction is launched between biotin labeled Thyroid Stimulating Hormone (TSH) and unlabeled Thyroid Stimulating Hormone (TSH) (Standards or samples) with the pre-coated antibody specific to Thyroid Stimulating Hormone (TSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thyroid Stimulating Hormone (TSH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Thyroid Stimulating Hormone (TSH) level in the sample or standard.

Alternative Names

Thyrotropin

Item Name

Thyroid Stimulating Hormone

Research Area

Endocrinology;Hormone metabolism;

Reference