CLIA Kit for Spermidine (SMD)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

781.25-200,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 284.75pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Spermidine (SMD). A competitive inhibition reaction is launched between biotin labeled Spermidine (SMD) and unlabeled Spermidine (SMD) (Standards or samples) with the pre-coated antibody specific to Spermidine (SMD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Spermidine (SMD) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Spermidine (SMD) level in the sample or standard.

Alternative Names

Item Name

Spermidine

Research Area

Reference