CLIA Kit for Luteinizing Hormone (LH)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

195-50,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 70pg/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Luteinizing Hormone (LH). A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone (LH) and unlabeled Luteinizing Hormone (LH) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone (LH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Luteinizing Hormone (LH) level in the sample or standard.

Alternative Names

ICSH; Lutropin; Interstitial Cell Stimulating Hormone

Item Name

Luteinizing Hormone

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Reference