CLIA Kit for Ischemia Modified Albumin (IMA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

370.4-30,000ng/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 131.3ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Ischemia Modified Albumin (IMA). A competitive inhibition reaction is launched between biotin labeled Ischemia Modified Albumin (IMA) and unlabeled Ischemia Modified Albumin (IMA) (Standards or samples) with the pre-coated antibody specific to Ischemia Modified Albumin (IMA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ischemia Modified Albumin (IMA) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Ischemia Modified Albumin (IMA) level in the sample or standard.

Alternative Names

Item Name

Ischemia Modified Albumin

Research Area

Metabolic pathway;Cardiovascular biology;

Reference