CLIA Kit for Hydroxymethylbilane Synthase (HMBS)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

68.6-50,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 25.8pg/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

2h, 40min

Method

Double-antibody Sandwich

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Hydroxymethylbilane Synthase (HMBS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hydroxymethylbilane Synthase (HMBS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hydroxymethylbilane Synthase (HMBS) level in the sample or standard.;

Alternative Names

PBG-D; PBGD; UPS; Hydroxymethylbilane Synthase; Uroporphyrinogen I Synthase; Porphobilinogen deaminase; Pre-uroporphyrinogen synthase

Item Name

Hydroxymethylbilane Synthase

Research Area

Metabolic pathway;

Reference