CLIA Kit for Heat Shock Protein 60 (Hsp60)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.14-100ng/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.04ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h, 40min

Method

Double-antibody Sandwich

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 60 (Hsp60). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Heat Shock Protein 60 (Hsp60). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Heat Shock Protein 60 (Hsp60) level in the sample or standard.;

Alternative Names

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

Item Name

Heat Shock Protein 60

Research Area

Signal transduction;Tumor immunity;

Reference