CLIA Kit for Growth Hormone (GH)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

16-10,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 5pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Growth Hormone (GH). A competitive inhibition reaction is launched between biotin labeled Growth Hormone (GH) and unlabeled Growth Hormone (GH) (Standards or samples) with the pre-coated antibody specific to Growth Hormone (GH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Growth Hormone (GH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Growth Hormone (GH) level in the sample or standard.

Alternative Names

GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

Item Name

Growth Hormone

Research Area

Endocrinology;Neuro science;Hormone metabolism;

Reference