CLIA Kit for Glycated Hemoglobin A1c (HbA1c)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

5.86-1,500μg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.44μg/mL

Sample type

serum, plasma and erythrocyte lysates

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Glycated Hemoglobin A1c (HbA1c). A competitive inhibition reaction is launched between biotin labeled Glycated Hemoglobin A1c (HbA1c) and unlabeled Glycated Hemoglobin A1c (HbA1c) (Standards or samples) with the pre-coated antibody specific to Glycated Hemoglobin A1c (HbA1c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glycated Hemoglobin A1c (HbA1c) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Glycated Hemoglobin A1c (HbA1c) level in the sample or standard.

Alternative Names

Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

Item Name

Glycated Hemoglobin A1c

Research Area

Reference