CLIA Kit for Collagen Type IV (COL4)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.14-100ng/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.07ng/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

2h, 40min

Method

Double-antibody Sandwich

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Collagen Type IV (COL4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Collagen Type IV (COL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Collagen Type IV (COL4) level in the sample or standard.;

Alternative Names

Type-IV Collagen

Item Name

Collagen Type IV

Research Area

Tumor immunity;Hepatology;

Reference

Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.;