CLIA Kit for Calcitroic Acid (CA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

195.3-50,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 75.1pg/mL

Sample type

Biological agents

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Calcitroic Acid (CA). A competitive inhibition reaction is launched between biotin labeled Calcitroic Acid (CA) and unlabeled Calcitroic Acid (CA) (Standards or samples) with the pre-coated antibody specific to Calcitroic Acid (CA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Calcitroic Acid (CA) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Calcitroic Acid (CA) level in the sample or standard.

Alternative Names

Item Name

Calcitroic Acid

Research Area

Reference