CLIA Kit for Beta Actin (ACTB)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.14-100ng/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.05ng/mL

Sample type

Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h, 40min

Method

Double-antibody Sandwich

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Beta Actin (ACTB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Beta Actin (ACTB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Beta Actin (ACTB) level in the sample or standard.;

Alternative Names

Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed

Item Name

Beta Actin

Research Area

Signal transduction;Developmental science;Bone metabolism;

Reference