CLIA Kit for Arginine (Arg)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.17-300ug/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.44ug/mL

Sample type

serum, plasma, tissue homogenates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Arginine (Arg). A competitive inhibition reaction is launched between biotin labeled Arginine (Arg) and unlabeled Arginine (Arg) (Standards or samples) with the pre-coated antibody specific to Arginine (Arg). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Arginine (Arg) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Arginine (Arg) level in the sample or standard.

Alternative Names

(S)-2-Amino-5-Guanidinopentanoic Acid

Item Name

Arginine

Research Area

Metabolic pathway;Nutrition metabolism;

Reference