CLIA Kit for Anti-Mullerian Hormone (AMH)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

61.73-5,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 22.84pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Anti-Mullerian Hormone (AMH). A competitive inhibition reaction is launched between biotin labeled Anti-Mullerian Hormone (AMH) and unlabeled Anti-Mullerian Hormone (AMH) (Standards or samples) with the pre-coated antibody specific to Anti-Mullerian Hormone (AMH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Anti-Mullerian Hormone (AMH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Anti-Mullerian Hormone (AMH) level in the sample or standard.

Alternative Names

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

Item Name

Anti-Mullerian Hormone

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Reference