CLIA Kit for Acetylcarnitine (ALCAR)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

19.5-5,000ng/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 7.4ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Acetylcarnitine (ALCAR). A competitive inhibition reaction is launched between biotin labeled Acetylcarnitine (ALCAR) and unlabeled Acetylcarnitine (ALCAR) (Standards or samples) with the pre-coated antibody specific to Acetylcarnitine (ALCAR). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Acetylcarnitine (ALCAR) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Acetylcarnitine (ALCAR) level in the sample or standard.

Alternative Names

Acetyl-L-carnitine

Item Name

Acetylcarnitine

Research Area

Metabolic pathway;Neuro science;Nutrition metabolism;

Reference