CHFR Antibody

Size

100ug

Clone Number

Aliases

Checkpoint with forkhead and ring finger domains antibody; Checkpoint with forkhead and RING finger domains protein antibody; Chfr antibody; CHFR_HUMAN antibody; E3 ubiquitin protein ligase CHFR antibody; E3 ubiquitin-protein ligase CHFR antibody; EC 6.3.2. antibody; FLJ10796 antibody; FLJ33629 antibody; RING finger protein 196 antibody; RNF 116 antibody; RNF 196 antibody; RNF116 antibody; RNF196 antibody; Ubiquitin ligase protein antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q96EP1

Immunogen

Recombinant Human E3 ubiquitin-protein ligase CHFR protein (141-422AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

Background

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the \\\'Lys-48\\\'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of \\\'Lys-63\\\'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at \\\'Lys-63\\\' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

CHFR

Research Areas

Epigenetics and Nuclear Signaling; Cancer; Cell biology