CEBPA Antibody

Size

100ug

Clone Number

Aliases

Apoptotic cysteine protease antibody; Apoptotic protease Mch 5 antibody; C/EBP alpha antibody; C/ebpalpha antibody; CAP4 antibody; Caspase 8 precursor antibody; CBF-A antibody; CCAAT Enhancer Binding Protein alpha antibody; CCAAT/enhancer binding protein (C/EBP); alpha antibody; CCAAT/enhancer-binding protein alpha antibody; CEBP antibody; CEBP A antibody; CEBP alpha antibody; Cebpa antibody; CEBPA_HUMAN antibody; FADD homologous ICE/CED 3 like protease antibody; FADD like ICE antibody; FLICE antibody; ICE like apoptotic protease 5 antibody; ICE8 antibody; MACH antibody; MCH5 antibody; MORT1 associated CED 3 homolog antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

P49715

Immunogen

Recombinant Human CCAAT/enhancer-binding protein alpha protein (1-124AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200

Background

Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5\\\'-T[TG]NNGNAA[TG]-3\\\' acting as an activator on distinct target genes (PubMed:11242107). During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Downregulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed:14660596). In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

CEBPA

Research Areas

Epigenetics and Nuclear Signaling; Cancer; Cardiovascular; Metabolism; Signal transduction