CDK4 Antibody

Size

100ul

Clone Number

8H4

Aliases

Cyclin-dependent kinase 4 (EC 2.7.11.22) (Cell division protein kinase 4) (PSK-J3), CDK4

Immunogen Species

Homo sapiens (Human)

UniProt ID

P11802

Immunogen

A synthesized peptide derived from human CDK4

Raised in

Species Reactivity

Human

Tested Applications

ELISA, WB; Recommended dilution: WB:1:500-1:5000

Background

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Clonality

Monoclonal

Isotype

Rabbit IgG

Purification Method

Affinity-chromatography

Conjugate

Non-conjugated

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

CDK4

Research Areas

Isotype/Loading Controls; Epigenetics and Nuclear Signaling; Cancer; Cell biology