CD14 Monoclonal Antibody

Size

100ul

Clone Number

1A7C5

Aliases

Monocyte differentiation antigen CD14 (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form], CD14

Immunogen Species

Human

UniProt ID

P08571

Immunogen

Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)

Raised in

Mouse

Species Reactivity

Human, Mouse, Rat, Rabbit

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:64000, IHC:1:50-1:200, IF:1:50-1:200

Background

Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187).

Clonality

Monoclonal

Isotype

IgG1

Purification Method

>95%, Protein A purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

CD14

Research Areas

Immunology; Stem cells