CARM1 Antibody

Size

100ug

Clone Number

Aliases

carm1 antibody; CARM1_HUMAN antibody; Coactivator associated arginine methyltransferase 1 antibody; Coactivator-associated arginine methyltransferase 1 antibody; Histone arginine methyltransferase CARM 1 antibody; Histone arginine methyltransferase CARM1 antibody; Histone-arginine methyltransferase CARM1 antibody; PRMT 4 antibody; PRMT4 antibody; Protein arginine methyltransferase antibody; Protein arginine N methyltransferase 4 antibody; Protein arginine N-methyltransferase 4 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q86X55

Immunogen

Recombinant Human Histone-arginine methyltransferase CARM1 protein (209-379AA)

Raised in

Rabbit

Species Reactivity

Human, Mouse

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Background

Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at \'Arg-17\' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at \'Arg-2142\', which may loosen its interaction with NCOA2/GRIP1, and at \'Arg-580\' and \'Arg-604\' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

CARM1

Research Areas

Epigenetics and Nuclear Signaling; Cancer; Cell biology; Metabolism; Signal transduction