Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

Host

Clone Number

Ig Isotype

Organism species

Oryctolagus cuniculus (Rabbit)

Concentration

500µg/mL

Application

WB; IHC; ICC.

Alternative Names

DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

Item Names

Tumor Necrosis Factor Alpha

Research Area

Cytokine;Tumor immunity;Infection immunity;

Citation

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Immunogen

PAA133Rb51-Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

Usage

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Traits

Liquid

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography