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SKU:BEK1344
MouseB7-2/CD86 ELISA Kit
MouseB7-2/CD86 ELISA Kit
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Application: For quantitative detection of B7-2 / CD86 in mouse serum, plasma, body fluids, tissue lysatesor cell culture supernatants.
Introduction: Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein constitutively expressed on dendritic cells, Langerhans cells, macrophages, B-cells (including memory B-cells), and on other antigen-presenting cells.Along with CD80, CD86 provides costimulatory signals necessary for T cell activation and survival. Depending on the ligand bound, CD86 can signal for self-regulation and cell-cell association, or for attenuation of regulation and cell-cell disassociation.The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily.Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-B7-2 / CD86 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-B7-2 / CD86 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theB7-2 / CD86amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofB7-2 / CD86can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 62.5pg/ml - 4000 pg/ml
Sensitivity: <10 pg/ml
Introduction: Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein constitutively expressed on dendritic cells, Langerhans cells, macrophages, B-cells (including memory B-cells), and on other antigen-presenting cells.Along with CD80, CD86 provides costimulatory signals necessary for T cell activation and survival. Depending on the ligand bound, CD86 can signal for self-regulation and cell-cell association, or for attenuation of regulation and cell-cell disassociation.The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily.Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-B7-2 / CD86 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-B7-2 / CD86 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theB7-2 / CD86amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofB7-2 / CD86can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 62.5pg/ml - 4000 pg/ml
Sensitivity: <10 pg/ml
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