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SKU:BEK1340
Human BCAM ELISA Kit
Human BCAM ELISA Kit
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$500.00 USD
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Application: For quantitative detection of BCAM in human serum, plasma, body fluids, tissue lysatesor cell culture supernatants.
Introduction: Basal cell adhesion molecule, also known as Lutheran antigen, is a plasma membrane glycoprotein that in humans is encoded by the BCAM gene.BCAM has also recently been designated CD239 (cluster of differentiation 239). Lutheran blood group glycoprotein is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five, N-terminus, extracellular immunoglobulin domains, a single transmembrane domain, and a short, C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BCAM polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BCAM polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theBCAMamount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofBCAMcan be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 156 pg/ml - 10,000 pg/ml
Sensitivity: <40 pg/ml
Introduction: Basal cell adhesion molecule, also known as Lutheran antigen, is a plasma membrane glycoprotein that in humans is encoded by the BCAM gene.BCAM has also recently been designated CD239 (cluster of differentiation 239). Lutheran blood group glycoprotein is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five, N-terminus, extracellular immunoglobulin domains, a single transmembrane domain, and a short, C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BCAM polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BCAM polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theBCAMamount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofBCAMcan be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 156 pg/ml - 10,000 pg/ml
Sensitivity: <40 pg/ml
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