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SKU:BEK1317
Human Pro-Cathepsin B ELISA Kit
Human Pro-Cathepsin B ELISA Kit
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Application: For quantitative detection of Pro-Catheps in B in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction: Cathepsin B belongs to a family of lysosomal cysteine proteases known as the cysteine cathepsins and plays an important role in intracellular proteolysis.In human s, cathepsin B is encoded by the CTSB gene.Cathepsin B is upregulated in certain cancers, in pre-malignant lesions, and in various other pathological conditions.The CTSB gene is located at chromosome 8p22, consisting of 13 exons. The promoter of CTSB gene contains a GC-rich region including many SP1 sites, which is similar to housekeeping genes. At least five transcript variants encoding the same protein have been found for this gene.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Pro-Cathepsin B polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Pro-Cathepsin B polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Pro-Cathepsin B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Pro-Cathepsin B can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 156 pg/ml - 10,000 pg/ml
Sensitivity: < 2 pg/ml
Introduction: Cathepsin B belongs to a family of lysosomal cysteine proteases known as the cysteine cathepsins and plays an important role in intracellular proteolysis.In human s, cathepsin B is encoded by the CTSB gene.Cathepsin B is upregulated in certain cancers, in pre-malignant lesions, and in various other pathological conditions.The CTSB gene is located at chromosome 8p22, consisting of 13 exons. The promoter of CTSB gene contains a GC-rich region including many SP1 sites, which is similar to housekeeping genes. At least five transcript variants encoding the same protein have been found for this gene.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Pro-Cathepsin B polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Pro-Cathepsin B polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Pro-Cathepsin B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Pro-Cathepsin B can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 156 pg/ml - 10,000 pg/ml
Sensitivity: < 2 pg/ml
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