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SKU:BEK1287
Human IL-23 ELISA Kit
Human IL-23 ELISA Kit
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Application: For quantitative detection of IL-23 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction: Interleukin-23 subunit alpha(IL-23) is a 189-amino acid protein that in human s is encoded by the IL23A gene. It is composed of the IL12 p40 subunit and the p19 subunit (IL23A), and is produced by dendritic cells and macrophages. Moreover, IL-23 is stimulated by Danger Signals, including cell debris, and directs memory T cells to Th17 response. It is an important part of the inflammatory response against infection. Recently, IL-23 has been implicated in the development of cancerous tumors. Langowski et al. (2006) showed that IL23 is an important molecular link between tumor-promoter proinflammatory processes and the failure of the adaptive immune surveillance to infiltrate tumors.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-23 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-23 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-23 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-23 can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 156 pg/ml - 10,000 pg/ml
Sensitivity: < 25 pg/ml
Introduction: Interleukin-23 subunit alpha(IL-23) is a 189-amino acid protein that in human s is encoded by the IL23A gene. It is composed of the IL12 p40 subunit and the p19 subunit (IL23A), and is produced by dendritic cells and macrophages. Moreover, IL-23 is stimulated by Danger Signals, including cell debris, and directs memory T cells to Th17 response. It is an important part of the inflammatory response against infection. Recently, IL-23 has been implicated in the development of cancerous tumors. Langowski et al. (2006) showed that IL23 is an important molecular link between tumor-promoter proinflammatory processes and the failure of the adaptive immune surveillance to infiltrate tumors.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-23 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-23 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-23 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-23 can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 156 pg/ml - 10,000 pg/ml
Sensitivity: < 25 pg/ml
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