1
/
of
1
Biospes
SKU:BEK1112
Mouse IL-7 ELISA Kit
Mouse IL-7 ELISA Kit
Regular price
$500.00 USD
Regular price
Sale price
$500.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Application: For quantitative detection of IL-7 in mouse serum, plasma,tissue lysates or cell culture supernatants.
Introduction: Interleukin 7(IL-7) is a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. Il-7 promotes hematological malignacies (acute lymphoblastic leukemia, T cell lymphoma), and the elevated levels of IL-7 have also been detected in the plasma of HIV-infected patients.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-7 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-7 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washedaway with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-7 can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: < 1 pg/ml
Introduction: Interleukin 7(IL-7) is a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. Il-7 promotes hematological malignacies (acute lymphoblastic leukemia, T cell lymphoma), and the elevated levels of IL-7 have also been detected in the plasma of HIV-infected patients.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-7 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-7 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washedaway with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-7 can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: < 1 pg/ml
Share
