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SKU:BEK1095
Mouse IL-1β ELISA Kit
Mouse IL-1β ELISA Kit
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Application: For quantitative detection of IL-1β in mouse serum,plasma, urine, cell culture supernatant or tissue samples.
Introduction: Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. It can reset glucose homeostasis at central levels. It cause oligodendrocyte death in coculture with astrocytes and microglia, and also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Pathak et al. (2011) proposed that IL1B blockade may be a viable therapy for patients with autoimmune inner ear disease or sensorineural hearing loss that fail to respond to corticosteroids.
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-IL-1β antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-IL-1βantibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1β amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1β can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months.
Range: 5 pg/ml - 100 pg/ml
Sensitivity:
Introduction: Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. It can reset glucose homeostasis at central levels. It cause oligodendrocyte death in coculture with astrocytes and microglia, and also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Pathak et al. (2011) proposed that IL1B blockade may be a viable therapy for patients with autoimmune inner ear disease or sensorineural hearing loss that fail to respond to corticosteroids.
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-IL-1β antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-IL-1βantibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1β amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1β can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months.
Range: 5 pg/ml - 100 pg/ml
Sensitivity:
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