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SKU:BEK1039
Human Cystatin C ELISA Kit
Human Cystatin C ELISA Kit
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$500.00 USD
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Application: For quantitative detection of Cysta in C in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction: Cystatin Cor cystatin 3, a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. It is a single 120-residue polypeptide with a molecular mass of approximately 13.26 kD.Cystatin Cplays a role in network reorganization in the epileptic dentate gyrus, especially in granule cell dispersion and guidance of migrating newborn granule cells.Itis an alternative measure of kidney function that may have prognostic importance among elderly persons who do not meet standard criteria for chronic kidney disease. Among elderly persons without chronic kidney disease,Cystatin Cis a prognostic biomarker of risk for death, cardiovascular disease, and chronic kidney disease, thereby signaling a state of 'preclinical' kidney dysfunction.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Cystatin C polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Cystatin C polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Cystatin C amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Cystatin C can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 312 pg/ml - 20,000 pg/ml
Sensitivity: < 10 pg/ml
Introduction: Cystatin Cor cystatin 3, a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. It is a single 120-residue polypeptide with a molecular mass of approximately 13.26 kD.Cystatin Cplays a role in network reorganization in the epileptic dentate gyrus, especially in granule cell dispersion and guidance of migrating newborn granule cells.Itis an alternative measure of kidney function that may have prognostic importance among elderly persons who do not meet standard criteria for chronic kidney disease. Among elderly persons without chronic kidney disease,Cystatin Cis a prognostic biomarker of risk for death, cardiovascular disease, and chronic kidney disease, thereby signaling a state of 'preclinical' kidney dysfunction.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Cystatin C polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Cystatin C polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Cystatin C amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Cystatin C can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 312 pg/ml - 20,000 pg/ml
Sensitivity: < 10 pg/ml
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