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SKU:BEK1002
Human Activin A ELISA Kit
Human Activin A ELISA Kit
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$500.00 USD
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Application: For quantitative detection of Activ in A in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction: Activin A, the homodimer of the beta-A subunit, is a cytokine member of the transforming growth factor-beta superfamily. It is produced in the gonads, pituitary gland, placenta, and other organs, and is expressed locally by the mesenchymal component of the hemopoietic microenvironment. It had a mitogenic effect on mouse osteoblastic cells and suppressed their alkaline phosphatase activity. It also has an important role in the inflammatory response and that FST may have significant therapeutic potential to reduce the severity of inflammatory diseases.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Activin A polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Activin A polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Activin A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Activin A can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: < 7.8 pg/ml
Introduction: Activin A, the homodimer of the beta-A subunit, is a cytokine member of the transforming growth factor-beta superfamily. It is produced in the gonads, pituitary gland, placenta, and other organs, and is expressed locally by the mesenchymal component of the hemopoietic microenvironment. It had a mitogenic effect on mouse osteoblastic cells and suppressed their alkaline phosphatase activity. It also has an important role in the inflammatory response and that FST may have significant therapeutic potential to reduce the severity of inflammatory diseases.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Activin A polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Activin A polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Activin A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Activin A can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: < 7.8 pg/ml
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