ATP5B Antibody

Size

100ul

Clone Number

5F10

Aliases

ATP synthase subunit beta, mitochondrial (EC 3.6.3.14), ATP5B, ATPMB ATPSB

Immunogen Species

Homo sapiens (Human)

UniProt ID

P06576

Immunogen

A synthesized peptide derived from human ATPB

Raised in

Species Reactivity

Human, Mouse, Rat

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200

Background

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Clonality

Monoclonal

Isotype

Rabbit IgG

Purification Method

Affinity-chromatography

Conjugate

Non-conjugated

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

ATP5B

Research Areas

Cancer; Tags & Cell Markers; Metabolism; Signal transduction