anti- RAD1 antibody

Host

Rabbit

Uniprot ID

O60671

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER). The 9-1-1 complex stimulates DNA polymerase beta(POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I(LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks(DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.

Immunogen

RAD1 homolog(S. pombe)

Synonyms

REC1

Observed MW

32 kDa

Reactivity

Human, Mouse, Rat

Tested Application

ELISA, WB, IHC, IP

Recommended dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

Gene ID

5810

Research Area

Epigenetics, Metabolism