anti- PTEN antibody

Host

Mouse

Uniprot ID

P60484

Form

liquid

Purification

Protein A+G purification

Purity

? 95% as determined by SDS-PAGE

Clonality

monoclonal

Clone ID

9000000

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Immunogen

phosphatase and tensin homolog

Synonyms

MMAC1, TEP1

Observed MW

47 kDa

Reactivity

Human, Mouse

Tested Application

ELISA, IHC, WB, IF

Recommended dilution

WB: 1:1000-1:4000; IHC: 1:200-1:1000; IF: 1:20-1:200

Gene ID

5728

Research Area

Signal Transduction, Metabolism, Cardiovascular, Immunology, Cancer, Neuroscience, Stem Cells, Cell Division and Proliferation