anti- POLR2D antibody

Host

Rabbit

Uniprot ID

O15514

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA(By similarity).

Immunogen

polymerase(RNA) II(DNA directed) polypeptide D

Synonyms

Observed MW

16-20 kDa

Reactivity

Human, Mouse, Rat

Tested Application

ELISA, WB, IP

Recommended dilution

WB: 1:500-1:2000; IP: 1:200-1:2000

Gene ID

5433

Research Area

Epigenetics, Metabolism