anti- POLR1A antibody

Host

Rabbit

Uniprot ID

O95602

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition(By similarity).

Immunogen

polymerase(RNA) I polypeptide A, 194kDa

Synonyms

A190, POLR1A, RNA polymerase I subunit A1, RPA1, RPA194, RPO1 4

Observed MW

190-195 kDa

Reactivity

Human

Tested Application

ELISA, WB

Recommended dilution

WB: 1:500-1:2000

Gene ID

25885

Research Area

Metabolism