anti- phospho(409/410)-TDP43 antibody

Host

Rabbit

Uniprot ID

Q13148

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

TDP-43 is a 414 amino acid (aa) nuclear DNA and RNA-binding protein which regulates transcription and splicing. TDP-43 is aberrantly expressed in most forms of amylotrophic lateral sclerosis and frontotemporal lobar degeneration. In these cases it is absent from the nucleus and accumulates, along with a truncated 25kDa form, in ubiquitin-containing inclusion bodies. In pathological tissues, TDP-43 is highly phosphorylated and Ser409/410 is the main phosphorylation site. Among the TDP-43 protein fragments, the 25kDa fragment is also highly phosphorylated and widely believed to be pathogenic. This antibody is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. This antibody detects phosphorylated forms of TDP-43 as well as the cleavage product of 25 kDa.

Immunogen

TAR DNA binding protein

Synonyms

phospho TDP43, phospho(409/410)-TDP43, TARDBP, TDP43

Observed MW

48 kDa

Reactivity

Human, Mouse

Tested Application

ELISA, WB

Recommended dilution

WB: 1:500-1:2000

Gene ID

23435

Research Area

Neuroscience, Metabolism, Epigenetics