anti- PHF2 antibody

Host

Rabbit

Uniprot ID

O75151

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3(H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3(H3K4me3) at rDNA promoters and promotes expression of rDNA.

Immunogen

PHD finger protein 2

Synonyms

GRC5, JHDM1E, KIAA0662, PHD finger protein 2, PHF2

Observed MW

100 kDa, 150 kDa

Reactivity

Human

Tested Application

ELISA, WB, IHC, IP

Recommended dilution

WB: 1:200-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200

Gene ID

5253

Research Area

Epigenetics, Metabolism