anti- NR5A1 antibody

Host

Rabbit

Uniprot ID

Q13285

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine(By similarity). Binds phospholipids with a phosphatidylinositol(PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

Immunogen

nuclear receptor subfamily 5, group A, member 1

Synonyms

AD4BP, FTZF1, SF1

Observed MW

53 kDa

Reactivity

Human, Mouse, Rat

Tested Application

ELISA, WB, IHC, IP

Recommended dilution

WB: 1:500-1:5000; IP: 1:200-1:1000; IHC: 1:20-1:200

Gene ID

2516

Research Area

Immunology, Signal Transduction, Metabolism, Developmental biology