anti- NLRP1 antibody

Host

Rabbit

Uniprot ID

Q9C000

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

As the sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP1, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death(PubMed:22665479, PubMed:17418785). May be activated by muramyl dipeptide(MDP), a fragment of bacterial peptidoglycan, in a NOD2-dependent manner(PubMed:18511561). Contrary to its mouse ortholog, not activated by Bacillus anthracis lethal toxin(PubMed:19651869). It is unclear whether isoform 2 is involved in inflammasome formation. It is not cleaved within the FIIND domain, does not assemble into specks, nor promote IL1B release(PubMed:22665479). However, in an vitro cell-free system, it has been shown to be activated by MDP(PubMed:17349957). Binds ATP(PubMed:11113115, PubMed:15212762).

Immunogen

NLR family, pyrin domain containing 1

Synonyms

CARD7, CLR17.1, DEFCAP, DEFCAP L/S, DKFZp586O1822, KIAA0926, NAC, NALP1, NLRP1, PP1044, SLEV1, VAMAS1

Observed MW

166kd

Reactivity

Human

Tested Application

ELISA, IHC, WB

Recommended dilution

WB: 1:500-1:2000; IHC: 1:20-1:200

Gene ID

22861

Research Area

Immunology