anti- Nestin antibody

Host

Mouse

Uniprot ID

P48681

Form

liquid

Purification

Protein A+G purification

Purity

>= 95%

Clonality

polyclonal

Clone ID

4F9

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system(CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem(hES) and induced pluripotent stem(iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Immunogen

nestin

Synonyms

NES, Nestin

Observed MW

170 kDa

Reactivity

Human, Mouse, Rat

Tested Application

ELISA, WB, IHC, IF

Recommended dilution

WB: 1:500-1:2000; IHC: 1:20-1:1000; IF: 1:20-1:200

Gene ID

10763

Research Area

Cardiovascular, Immunology, Cancer, Developmental biology, Neuroscience, Stem Cells