anti- NEIL1 antibody

Host

Rabbit

Uniprot ID

Q96FI4

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine(Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP(apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'-and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine(5hmC), suggesting that it acts as a specific reader of 5hmC.

Immunogen

nei endonuclease VIII-like 1(E. coli)

Synonyms

DNA glycosylase/AP lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII like 1, FPG1, hFPG1, NEH1, Nei homolog 1, Nei like protein 1, NEI1, NEIL1

Observed MW

44kd

Reactivity

Human, Mouse, Rat

Tested Application

ELISA, WB

Recommended dilution

WB: 1:500-1:1000

Gene ID

79661

Research Area

Metabolism