anti- NDC80 antibody

Host

Rabbit

Uniprot ID

O14777

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity(PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore(PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules(PubMed:23085020).

Immunogen

NDC80 homolog, kinetochore complex component(S. cerevisiae)

Synonyms

HEC, HEC1, KNTC2

Observed MW

76 kDa

Reactivity

Human, Mouse, Rat

Tested Application

ELISA, WB, IHC, IP

Recommended dilution

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

Gene ID

10403

Research Area

Cell Division and Proliferation, Metabolism