anti- CD146/MCAM antibody

Host

Mouse

Uniprot ID

P43121

Form

liquid

Purification

Protein A+G purification

Purity

? 95% as determined by SDS-PAGE

Clonality

monoclonal

Clone ID

0

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

CD146, also known as melanoma cell adhesion molecule(MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein belonging to the immunoglobulin(Ig) superfamily(PMID: 8378324). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells(CECs)(PMID: 19356677).

Immunogen

melanoma cell adhesion molecule

Synonyms

CD146, MCAM, MUC18,Cell surface glycoprotein MUC18,Cell surface glycoprotein P1H12,Melanoma cell adhesion molecule,Melanoma-associated antigen A32,Melanoma-associated antigen MUC18

Observed MW

113-120 kDa

Reactivity

Human

Tested Application

ELISA, WB, IHC, FC

Recommended dilution

WB: 1:500-1:5000; IHC: 1:20-1:200

Gene ID

Research Area

Cardiovascular, Immunology, Cancer, Developmental biology, Stem Cells