anti- BRD8 antibody

Host

Mouse

Uniprot ID

Q9H0E9

Form

liquid

Purification

Protein A+G purification

Purity

? 95% as determined by SDS-PAGE

Clonality

monoclonal

Clone ID

4B2

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors(NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase(HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.

Immunogen

bromodomain containing 8

Synonyms

SMAP, SMAP2

Observed MW

120 kDa

Reactivity

Human

Tested Application

ELISA, WB, IHC, IF

Recommended dilution

WB: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500

Gene ID

10902

Research Area

Signal Transduction, Metabolism