anti- BORIS antibody

Host

Mouse

Uniprot ID

Q8NI51

Form

liquid

Purification

Protein A+G purification

Purity

? 95% as determined by SDS-PAGE

Clonality

monoclonal

Clone ID

2B10

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

CTCFL, also named BORIS(for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region(ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.

Immunogen

CCCTC-binding factor(zinc finger protein)-like

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, dJ579F20.2, Zinc finger protein CTCF T

Observed MW

75 kDa

Reactivity

Human, Mouse

Tested Application

ELISA, WB, IF

Recommended dilution

WB: 1:500-1:2000; IF: 1:10-1:100

Gene ID

Research Area

Metabolism