anti- ATR antibody

Host

Rabbit

Uniprot ID

Q13535

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation(IR), ultraviolet light(UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

Immunogen

ataxia telangiectasia and Rad3 related

Synonyms

ATR, FRAP related protein 1, FRP1, MEC1, SCKL, SCKL1

Observed MW

250-290kd

Reactivity

Human, Mouse, Rat

Tested Application

ELISA, WB, IHC, IF

Recommended dilution

WB: 1:300-1:1000; IP: 1:200-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500

Gene ID

545

Research Area

Epigenetics, Metabolism, Developmental biology