anti- ATP5C1 antibody

Host

Mouse

Uniprot ID

P36542

Form

liquid

Purification

Protein A+G purification

Purity

? 95% as determined by SDS-PAGE

Clonality

monoclonal

Clone ID

8H11

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Mitochondrial membrane ATP synthase(F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1)-containing the extramembraneous catalytic core, and F(0)-containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Immunogen

ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1

Synonyms

ATP5C, ATP5C1, ATP5CL1, F ATPase gamma subunit

Observed MW

33 kDa

Reactivity

Human

Tested Application

ELISA, WB, IHC

Recommended dilution

WB: 1:500-1:2000; IHC: 1:100-1:500

Gene ID

509

Research Area

Metabolism