anti- ATP5A1 antibody

Host

Mouse

Uniprot ID

P25705

Form

liquid

Purification

Protein A+G purification

Purity

? 95% as determined by SDS-PAGE

Clonality

monoclonal

Clone ID

9G0

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Mitochondrial membrane ATP synthase(F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1)-containing the extramembraneous catalytic core, and F(0)-containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites(By similarity).

Immunogen

ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle

Synonyms

ATP5A, ATP5AL2, ATPM

Observed MW

50-55 kDa

Reactivity

Human, Mouse, Rat

Tested Application

ELISA, IHC, IF, WB, FC

Recommended dilution

WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

Gene ID

498

Research Area

Metabolism