anti- ATF4 antibody

Host

Mouse

Uniprot ID

P18848

Form

liquid

Purification

Protein A+G purification

Purity

? 95% as determined by SDS-PAGE

Clonality

monoclonal

Clone ID

0B11

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Transcriptional activator. Binds the cAMP response element(CRE)(consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production(By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase(ASNS) in response to endoplasmic reticulum(ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements(CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors(PubMed:26086088).

Immunogen

activating transcription factor 4

Synonyms

ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB

Observed MW

50-58 kDa

Reactivity

Human, Mouse

Tested Application

ELISA, IHC, WB, IF

Recommended dilution

WB: 1:1000-1:2000; IHC: 1:50-1:300; IF: 1:20-1:100

Gene ID

468

Research Area

Neuroscience, Epigenetics, Metabolism, Developmental biology