anti- APP antibody

Host

Mouse

Uniprot ID

P05067

Form

liquid

Purification

Protein A+G purification

Purity

? 95% as determined by SDS-PAGE

Clonality

monoclonal

Clone ID

8B9

Isotype

IgG2a

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.

Immunogen

amyloid beta A4 precursor protein

Synonyms

A4, ABPP,Alzheimer disease amyloid protein,Cerebral vascular amyloid peptide,Beta-amyloid precursor protein, CVAP,PreA4

Observed MW

100 kDa

Reactivity

Human, Pig

Tested Application

ELISA, WB, IHC

Recommended dilution

WB: 1:500-1:2000; IHC: 1:50-1:500

Gene ID

351

Research Area

Neuroscience, Signal Transduction, Metabolism