anti- Amphiregulin antibody

Host

Rabbit

Uniprot ID

P15514

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

Amphiregulin(AREG) is one of the ligands of the epidermal growth factor receptor(EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms(PMID: 9642297).

Immunogen

amphiregulin

Synonyms

Amphiregulin, AR, AREG, AREGB, CRDGF, SDGF

Observed MW

28 kDa, observed 43 kDa

Reactivity

Human

Tested Application

ELISA, IF, WB, IHC, FC, IP

Recommended dilution

WB: 1:200-1:2000; IHC: 1:50-1:500; IP: 1:1000-1:10000; IF: 1:10-1:100

Gene ID

374

Research Area

Immunology, Cardiovascular, Signal Transduction