anti- AMFR antibody

Host

Rabbit

Uniprot ID

Q9UKV5

Form

liquid

Purification

Immunogen affinity purified

Purity

>= 95%

Clonality

polyclonal

Clone ID

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Background

E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation(ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.

Immunogen

autocrine motility factor receptor

Synonyms

AMF receptor, isoform 2, AMFR, GP78, RING finger protein 45, RNF45

Observed MW

73 kDa

Reactivity

Human, Mouse, Rat

Tested Application

ELISA, WB, IHC, IF, IP

Recommended dilution

WB: 1:500-1:5000; IF: 1:10-1:100; IHC: 1:20-1:200; IP: 1:200-1:2000

Gene ID

267

Research Area

Epigenetics, Signal Transduction, Metabolism